Journal: Differentiation; research in biological diversity

Article Title: The combination of glycosaminoglycans and fibrous proteins improves cell proliferation and early differentiation of bovine primary skeletal muscle cells.

doi: 10.1016/j.diff.2013.06.006

Figure Lengend Snippet: Fig. 2. Localization and expression of myogenic regulatory transcription factors (MRFs) during proliferation and differentiation. (A) Proliferating cells were fixed with 2% PFA, immunostained with Alexa 488-Phalloidin (green), and rabbit anti-MyoD, followed by DyLight 649-conjugated donkey anti-rabbit (red) before fluorescence microscopy analysis. Nuclei were stained with DAPI (blue). Scale bar 10 mM. (B) Cells differentiated for 3 days were fixed with 2% PFA, immunostained with Alexa 488-Phalloidin (green), and goat anti-Myogenin, followed by DyLight 649-conjugated donkey anti-goat (red) before fluorescence microscopy analysis. Nuclei were stained with DAPI (blue). Arrows indicate Myogenin positive cells. Scale bar 10 mM. (C) Differentiating cells were fixed with 2% PFA, immunostained with Alexa 488-Phalloidin (green) and rabbit anti-Desmin, followed by DyLight 549-conjugated mouse anti-rabbit (red) before fluorescence microscopy analysis. Nuclei were stained with DAPI (blue). Scale bar 10 mM. (D) Left: a representative western blot showing the expression level of MyoD and Desmin after 0 and 3 days of differentiation. Cell lysates were subjected to western blotting using antibodies to MyoD, Desmin, and Tubulin (loading control). Right: quantification of Western blots. Bars show the expression of Desmin and MyoD, relative to tubulin, after 0 and 3 days of differentiation. The graph represents the average of three experiments with SD.

Article Snippet: Alexa 488-conjugated goat anti-mouse and DyLight 549- conjugated mouse anti-rabbit were from Jackson ImmunoResearch Laboratories Inc. (West Grove, PA, USA).

Techniques: Expressing, Microscopy, Staining, Western Blot, Control

Journal: Differentiation; research in biological diversity

Article Title: The combination of glycosaminoglycans and fibrous proteins improves cell proliferation and early differentiation of bovine primary skeletal muscle cells.

doi: 10.1016/j.diff.2013.06.006

Figure Lengend Snippet: Fig. 6. Desmin staining of myotubes on different surface coatings after 1, 3 or 5 days in differentiation medium. Differentiating cells were fixed with 2% PFA and immunostained with rabbit anti-Desmin, followed by DyLight 549-conjugated mouse anti-rabbit (yellow) before fluorescence microscopy analysis. Nuclei were stained with DAPI (blue). Scale bar 20 mM.

Article Snippet: Alexa 488-conjugated goat anti-mouse and DyLight 549- conjugated mouse anti-rabbit were from Jackson ImmunoResearch Laboratories Inc. (West Grove, PA, USA).

Techniques: Staining, Microscopy